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room temperature  (Vector Laboratories)


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    Vector Laboratories room temperature
    Room Temperature, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 2773 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+on+mouse+(m%2Eo%2Em%2E+)+basic+kit/Mouse+on+Mouse+(M%2EO%2EM%2E+)+Basic+Kit/bio_rxiv__64898__2026__04__21__719997-92-20-13
    Average 96 stars, based on 2773 article reviews
    room temperature - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: ASO-based PKM splice-switching therapy increases anti-CTLA-4 antibody efficacy in pancreatic ductal adenocarcinoma.
    Article Snippet: For IF, the signal was visualized with fluoro-conjugated secondary antibodies (Thermo Fisher Scientific, goat anti-mouse immunoglobulin G (IgG) (H + L) Highly Cross-Adsorbed, Alexa Fluor Plus 555, A32727 or goat antirabbit IgG (H + L) Highly Cross-Adsorbed, Alexa Fluor Plus 488, A32731). .. Before being incubated with the mouse-derived anti-ASO antibody, mouse tissues were treated with an M.O.M. kit (Vector Labs, BMK-2202) to reduce endogenous mouse IgG staining, and the ASO was visualized with a streptavidin and Alexa Fluor 555 Conjugate (Thermo Fisher Scientific, S21381). .. Slides were counterstained with 0.1 μg/mL DAPI (Thermo Fisher Scientific; USA), and images were captured on a Zeiss LSM710 confocal laser scanning microscope. scRNA-seq and statistical analysis The scRNA-seq data were analyzed as previously described47.

    Article Title: ASO-based PKM splice-switching therapy increases anti-CTLA-4 antibody efficacy in pancreatic ductal adenocarcinoma
    Article Snippet: For IF, the signal was visualized with fluoro-conjugated secondary antibodies (Thermo Fisher Scientific, goat anti-mouse immunoglobulin G (IgG) (H + L) Highly Cross-Adsorbed, Alexa Fluor Plus 555, A32727 or goat anti-rabbit IgG (H + L) Highly Cross-Adsorbed, Alexa Fluor Plus 488, A32731). .. Before being incubated with the mouse-derived anti-ASO antibody, mouse tissues were treated with an M.O.M. kit (Vector Labs, BMK-2202) to reduce endogenous mouse IgG staining, and the ASO was visualized with a streptavidin and Alexa Fluor 555 Conjugate (Thermo Fisher Scientific, S21381). .. Slides were counterstained with 0.1 μg/mL DAPI (Thermo Fisher Scientific; USA), and images were captured on a Zeiss LSM710 confocal laser scanning microscope.

    Article Title: C26 and CT26 colorectal cancer models exhibit divergent cachexia phenotypes, intramuscular inflammation, and protein turnover signaling
    Article Snippet: .. All the slides used for immunostaining were blocked in M.O.M immunoglobulin blocking reagent (Vector Laboratories, BMK-2202) for 1 hour at room temperature before overnight incubation at 4°C with primary antibodies. ..

    Staining:

    Article Title: ASO-based PKM splice-switching therapy increases anti-CTLA-4 antibody efficacy in pancreatic ductal adenocarcinoma.
    Article Snippet: For IF, the signal was visualized with fluoro-conjugated secondary antibodies (Thermo Fisher Scientific, goat anti-mouse immunoglobulin G (IgG) (H + L) Highly Cross-Adsorbed, Alexa Fluor Plus 555, A32727 or goat antirabbit IgG (H + L) Highly Cross-Adsorbed, Alexa Fluor Plus 488, A32731). .. Before being incubated with the mouse-derived anti-ASO antibody, mouse tissues were treated with an M.O.M. kit (Vector Labs, BMK-2202) to reduce endogenous mouse IgG staining, and the ASO was visualized with a streptavidin and Alexa Fluor 555 Conjugate (Thermo Fisher Scientific, S21381). .. Slides were counterstained with 0.1 μg/mL DAPI (Thermo Fisher Scientific; USA), and images were captured on a Zeiss LSM710 confocal laser scanning microscope. scRNA-seq and statistical analysis The scRNA-seq data were analyzed as previously described47.

    Article Title: ASO-based PKM splice-switching therapy increases anti-CTLA-4 antibody efficacy in pancreatic ductal adenocarcinoma
    Article Snippet: For IF, the signal was visualized with fluoro-conjugated secondary antibodies (Thermo Fisher Scientific, goat anti-mouse immunoglobulin G (IgG) (H + L) Highly Cross-Adsorbed, Alexa Fluor Plus 555, A32727 or goat anti-rabbit IgG (H + L) Highly Cross-Adsorbed, Alexa Fluor Plus 488, A32731). .. Before being incubated with the mouse-derived anti-ASO antibody, mouse tissues were treated with an M.O.M. kit (Vector Labs, BMK-2202) to reduce endogenous mouse IgG staining, and the ASO was visualized with a streptavidin and Alexa Fluor 555 Conjugate (Thermo Fisher Scientific, S21381). .. Slides were counterstained with 0.1 μg/mL DAPI (Thermo Fisher Scientific; USA), and images were captured on a Zeiss LSM710 confocal laser scanning microscope.

    Allele-specific Oligonucleotide:

    Article Title: ASO-based PKM splice-switching therapy increases anti-CTLA-4 antibody efficacy in pancreatic ductal adenocarcinoma.
    Article Snippet: For IF, the signal was visualized with fluoro-conjugated secondary antibodies (Thermo Fisher Scientific, goat anti-mouse immunoglobulin G (IgG) (H + L) Highly Cross-Adsorbed, Alexa Fluor Plus 555, A32727 or goat antirabbit IgG (H + L) Highly Cross-Adsorbed, Alexa Fluor Plus 488, A32731). .. Before being incubated with the mouse-derived anti-ASO antibody, mouse tissues were treated with an M.O.M. kit (Vector Labs, BMK-2202) to reduce endogenous mouse IgG staining, and the ASO was visualized with a streptavidin and Alexa Fluor 555 Conjugate (Thermo Fisher Scientific, S21381). .. Slides were counterstained with 0.1 μg/mL DAPI (Thermo Fisher Scientific; USA), and images were captured on a Zeiss LSM710 confocal laser scanning microscope. scRNA-seq and statistical analysis The scRNA-seq data were analyzed as previously described47.

    Article Title: ASO-based PKM splice-switching therapy increases anti-CTLA-4 antibody efficacy in pancreatic ductal adenocarcinoma
    Article Snippet: For IF, the signal was visualized with fluoro-conjugated secondary antibodies (Thermo Fisher Scientific, goat anti-mouse immunoglobulin G (IgG) (H + L) Highly Cross-Adsorbed, Alexa Fluor Plus 555, A32727 or goat anti-rabbit IgG (H + L) Highly Cross-Adsorbed, Alexa Fluor Plus 488, A32731). .. Before being incubated with the mouse-derived anti-ASO antibody, mouse tissues were treated with an M.O.M. kit (Vector Labs, BMK-2202) to reduce endogenous mouse IgG staining, and the ASO was visualized with a streptavidin and Alexa Fluor 555 Conjugate (Thermo Fisher Scientific, S21381). .. Slides were counterstained with 0.1 μg/mL DAPI (Thermo Fisher Scientific; USA), and images were captured on a Zeiss LSM710 confocal laser scanning microscope.

    Immunostaining:

    Article Title: C26 and CT26 colorectal cancer models exhibit divergent cachexia phenotypes, intramuscular inflammation, and protein turnover signaling
    Article Snippet: .. All the slides used for immunostaining were blocked in M.O.M immunoglobulin blocking reagent (Vector Laboratories, BMK-2202) for 1 hour at room temperature before overnight incubation at 4°C with primary antibodies. ..

    Blocking Assay:

    Article Title: C26 and CT26 colorectal cancer models exhibit divergent cachexia phenotypes, intramuscular inflammation, and protein turnover signaling
    Article Snippet: .. All the slides used for immunostaining were blocked in M.O.M immunoglobulin blocking reagent (Vector Laboratories, BMK-2202) for 1 hour at room temperature before overnight incubation at 4°C with primary antibodies. ..

    Article Title: HIPK2-and IKKβ-dependent phosphorylation stabilizes TAp63α during the oocyte DNA damage response
    Article Snippet: .. Blocking was carried out using M.O.M (BMK-2202, vector labs) if using mouse monoclonal primary antibody for mouse tissue for 1hr at RT followed by blocking with ADB twice for 15 mins each. .. The sections were incubated overnight with primary antibodies: [Mouse anti-TAp63α (Cat no. CM163B, 1:500), Rabbit anti-MVH (Cat no. ab13840, Abcam 1:500), Rabbit anti-Cleaved Caspase 3 (Cat no. PA5-114687, Thermo, 1:200), Mouse anti γH2A.X (05-636, Millipore,1:200)].



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